Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Seracare Life Sciences Inc 10X TRIS-GLYCINE-SDS BUFFER
Tris-Glycine-SDS (TGS) is a running buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. pH=8.3. Conductivity controlled.
![Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659834.jpg-250.jpg)
Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 100 Grams
BIS TRIS Propane (1,3 Bis (TRIS(hydroxymethyl)methylamino)propane) is a biochemical reagent used as a buffering agent in laboratory research. The 100g quantity provides enough material for numerous experiments that require pH stability in a range of biological and chemical applications. BIS TRIS Propane is particularly valuable for creating buffers with a stable pH in the physiological range, making it ideal for protein studies, enzymatic assays, and nucleic acid work. It is commonly used in molecular biology, microbiology, and biochemistry for maintaining consistent conditions during reactions. This reagent is a reliable choice for labs that require precise control over pH to ensure the integrity of their experiments.
Bioworld TT Buffer, Ready-Pack (10X), 1 L
Tris-Tricine BufferTris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more conventionally used buffers. TT Buffer allows for optimal resolution of low molecular weight proteins and elimantes the glycine front often found in gel utilizing TG-SDS and other such beffers. This improved running buffer allows for resolution of peptides as low as 5kD. TT buffer is also excellent for protein electrophoresis where resolved products will be transferred to a nylon membrane. Unlike glycine, tricine will not interfere with membrane protein sequencing. The 10X pack just requires the addition of distilled H2O to make 1.0 Liter of 10X liquid. A simple 10-fold dilution with water provides a 1X solution, that makes an excellent running buffer for most applications. Technical Information:Ultra PureGradeStorage: RTHazard: Irritant
Enzo Life Sciences Ada-Tyr-(Ahx)3-(Leu)3-vinyl sulfone (100µg)
A highly potent, cell-permeable proteasome inhibitor that targets all individual active subunits with comparable affinity. This extended peptide-based inhibitor can be radioiodinated, allowing detection of labeled subunits by autoradiography. Purity: ≥97%. Long Term Storage: -20°C.
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660092.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 1 Kg
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a specialized biochemical reagent used for preparing buffer solutions that maintain a stable pH in laboratory experiments. This 1kg formulation is ideal for high-volume applications, including protein chemistry, enzyme assays, and microbiological studies. The sesquisodium salt form of PIPES offers enhanced buffering capacity and is commonly used in research that requires precise pH control, such as in cell culture, protein purification, and electrophoresis. The buffer solution maintains a stable pH around 7.0, which is essential for maintaining enzyme activity and cellular functions during experiments.
Bioworld Bicine, 500 g
A zwitterionic amino acid buffer agent used for low temperature work Technical Information:Synonym: N,N-Bis(2-hydroxyethyl)glycine Titration: > 98.0% (0.1N NaOH)Water content: < 2.0%Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.Appearance: PowderBoiling Point : 388.55 °C at 760 mmHgDensity : 1.05 g/cm3 at 20 °C (lit.)HS Code : 29225000Melting Point: 190 °C (dec.)(lit.)PH Value: 4.3-5.3 (1%)RTECS Number: MB9700000
Alkali Scientific ADA, [N (Carbamoylmethyl) iminodiacetic acid), 25 G
ADA (N-(Carbamoylmethyl) iminodiacetic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is ideal for laboratory applications requiring precise pH regulation. ADA is commonly used in chromatography, protein studies, and enzyme assays, providing a stable pH environment during various biochemical experiments. Its ability to maintain a controlled pH is essential for accurate results in protein purification, cell culture, and molecular biology studies. The 25g size is perfect for smaller-scale experiments or routine laboratory use where reliable buffering capacity is necessary for optimal results.
Crescent Chemical Co Inc MORPHOLINOPROPANE SULFONICACID
Morpholinopropane sulfonic acid analytical grade pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. Size - 100G Sto
![Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000409966-logo.png-250.jpg){kind=logo}
Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G
TAPS is an aminosulfonate-based lysozyme stabilizer and connexin channel inhibitor. It helps maintain the native structure of lysozyme in aqueous solution at pH 7.0 and protects it from heat-induced denaturation. TAPS is also considered a critical compound for investigating the structure and function of connexin channels.
- Maintains native structure of lysozyme in aqueous solution at pH 7.0
- Protects lysozyme from heat-induced denaturation
- Directly inhibits heteromeric connexin 32/connexin 26 channels (Cx32/Cx26)
- Reduces connexin channel-mediated solute exchange in a recombinant liposome system
- Critical compound for investigating connexin channel structure and function
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660293.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 100 G
MES Sodium Salt (2-(N-Morpholino) ethanesulfonic acid), sodium salt, in a 100g volume is a biochemical reagent designed for pH buffering in laboratory experiments. It is commonly used in biological assays, cell culture, and protein purification due to its ability to maintain a stable and non-interfering pH. The sodium salt form of MES is particularly useful in experiments where higher ionic strength is required. This 100g size is ideal for smaller experiments and research studies that need precise pH control without requiring large quantities of reagent. MES Sodium Salt is an essential component in molecular biology and biochemistry for maintaining optimal conditions in sensitive biological assays.
Alkali Scientific MES, Anhydrous, 1 Kg
MES, Anhydrous, 1 Kg, is a highly effective buffering reagent used in laboratories to maintain stable pH levels, particularly in biological, biochemical, and chemical applications. The anhydrous form of MES ensures that the buffer can be easily dissolved in water or other solvents for customized concentrations. It is primarily used in electrophoresis, enzyme reactions, and in preparing cell culture media. MES provides excellent buffering capacity in the pH range of 5.5 to 6.7, making it ideal for a variety of experiments that require precise pH control. The 1 kg weight is perfect for medium-scale laboratory use, offering both convenience and consistency in experiments.
Medchemexpress LLC Pipes (monosodium) | 10010-67-0 | 99.6% | 324.35 | 10 G
PIPES monosodium (1,4-Piperazinediethanesulfonic acid monosodium) is a pH buffer. It can be mixed with the disodium salt form of PIPES to adjust the pH and ionic strength of the medium by altering the ratio and total amounts of the buffers. This product is intended for research use only.
- Functions as a pH buffer
- Can be mixed with disodium salt form of PIPES
- Allows adjustment of pH and ionic strength
- Intended for research use only
Medchemexpress LLC HEPPSO | 68399-78-0 | 99.9% | 25 G
HEPPSO is a zwitterionic buffer designed for various research applications. It operates within a working pH range of 7.1-8.5 and exhibits a relatively high ability to bind copper(II). With a pKa of 7.84 at a 2.0 mM buffer concentration, it is suitable for use in laboratory settings.
- Zwitterionic buffer
- Working pH range of 7.1-8.5
- High ability to bind copper(II)
- pKa of 7.84 at 2.0 mM buffer concentration
- For research use only