Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 500 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 500 Tablets per Bottle
PBS [Phosphate Buffered Saline] 100 mL Tablets are designed to make buffer preparation simple and efficient. Each tablet dissolves easily in water to create 100 mL of phosphate-buffered saline. With 500 tablets per bottle, these tablets are perfect for laboratories with high-volume buffer preparation needs. The ease of use and consistency of the PBS solution make these tablets ideal for various applications, including cell culture, microbiological analysis, and molecular biology experiments. The tablets provide a reliable way to ensure that each PBS solution is accurately prepared with the correct pH and osmolarity, minimizing the potential for human error in buffer formulation.
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
Apexbio Technology LLC Annexin V-HF488/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes Propidium iodide (PI) selectively binds double-stranded DNA and yields a red fluorescence PI is membrane-impermeable in healthy cells but can penetrate the membranes of late apoptotic and dead cells staining their nuclei Simultaneous staining with Annexin V-HF488 and PI enables differentiation between early apoptotic late apoptotic and dead cells
Biotang Inc Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S
Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S.
Apexbio Technology LLC Protease Inhibitor Cocktail (100X in DMSO EDTA plus) 100x(1ml A 1ml B)
This product is a protease inhibitor cocktail (100X in DMSO EDTA plus) specifically designed for use during cell lysis and protein extraction processes It is a ready-to-use concentrated solution that can inhibit a variety of endogenous proteases Also included is a separate 0 5 M EDTA solution which effectively inhibits metalloproteases The inhibitor cocktail consists of six carefully optimized broad-spectrum protease inhibitors dissolved in DMSO capable of efficiently inhibiting serine proteases cysteine proteases aspartic proteases and aminopeptidases commonly present in cell lysates EDTA is provided for inhibition of metalloproteases If immobilized metal affinity chromatography (IMAC) is involved for protein purification or if two-dimensional (2D) gel electrophoresis analysis is planned EDTA must be thoroughly removed by extensive dialysis or desalting prior to purification Specification 1 Set equals 1X (1 mL A 1 mL B) 10 Sets equals 10X (1 mL A 1 mL B) and so on
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 6X500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (6 x 500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
ILEX LIFE SCIENCES LLC 2-MERCAPTOETHANOL 50MM 100ML
PAN-Biotech 2-Mercaptoethanol 50 mM in DPBS (100 ml). DESCRIPTION: 2-Mercaptoethanol, also known as beta-mercaptoethanol (BME), is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, thus protocols generally require daily supplementation. PAN-Biotech 2-Mercaptoethanol is supplied at a concentration of 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). SOLUTION: 50 mM in DPBS. STORAGE: +2-8°C. STERILE: Yes. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Teknova 1X PBS-TBN Buffer with 0.05% Tween, 1% BSA, 0.1% Sodium Azide. 1000mL, Sterile. 4 pack.
150mM Sodium Chloride, 50mM Monosodium Phosphate,1.00% Bovine Serum Albumin, 0.10% Sodium Azide,0.05% Tween-20 (v/v)
Alkali Scientific CellPro™ 1X Balanced Salt Solution, 500ml
Balanced salt solution for cell culture applications. All formulations incorporate a rigorous specification and approval process. Available in lot sizes that facilitate scale-up and manufacturing.
ILEX LIFE SCIENCES LLC TRYPSIN 10X P10-022100 6X100ML
PAN-Biotech (10x) Trypsin 2.5 % in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 2.5% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in a 10x concentrated PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 2.50% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Bioworld PBS-Casein, 0.5%, 200 mL
Supplied in PBS as 0.5% casein ready for use.pH range 7.4 (+/- 0.1)Sold as 2 x 100mL or 500mL.
Ethos Biosciences Dilurex 1X PBS Buffer, 1000ml, Ethos
Dilurex 1X PBS is a ready-to-use working concentration of pH 7.4 phosphate buffered saline. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 1X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
Gold Biotechnology Inc HEPES Free Acid 2 x 1 kg
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions