Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-20 Bis-Tris Preca
mPAGE 4-20% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Teknova 50X TAE, Electrophoresis Grade. (2M Tris-Acetate, 100mM EDTA) 1000mL, Non-Sterile. 2 pack.
2M Tris-Acetate, 100mM EDTA
eMolecules TAE buffer | 610769-36-3 | MFCD00236357 | 25g
Combi-Blocks, Inc. | TAE buffer | 25g | 586078156 | QF-5924 | 95.000 | 610769-36-3 | MFCD00236357 | 473.432 | C16H31N3O13
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool
![eMolecules tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.2.2]octane-2-carboxylate | 2306265-70-1 | | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502159806.JPG-250.jpg)
eMolecules tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.2.2]octane-2-carboxylate | 2306265-70-1 | | 1g
Pharmablock | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.2.2]octane-2-carboxylate | 1g | 596622780 | PBV0121 | | 2306265-70-1 | | 241.331 | C13H23NO3
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool
Medchemexpress LLC P62-mediated mitopha 10mM 1mL | 1809031-84-2 | 392.15 g·mol⁻1 | C14H9IN4O2 | 1 ML
P62-mediated mitophagy inducer is a small-molecule research reagent that activates p62-mediated mitophagy without recruiting Parkin or collapsing mitochondrial membrane potential. It is supplied as a 10 mM stock solution (1 mL) for use in cellular and biochemical studies of selective mitochondrial clearance and autophagy pathways.
- Activates p62-mediated mitophagy without Parkin recruitment.
- Provided as a ready-to-use 10 mM stock solution in 1 mL.
- Suitable for cellular and biochemical assays investigating mitophagy and autophagy.
- Stable storage recommendations: powder at -20°C for up to 3 years; in solvent at -80°C or -20°C as indicated.
- Comes with chemical identification and characterization data (CAS, formula, molecular weight) in the supplier datasheet.
National Diagnostics ProtoGel Resolving Buffer (4X) 450ML
Traditional Laemmli Resolving Gel Buffer (4X). Formulated with 0.2 Micron Filtration.Ultra-Pure Reagents in 18 MegOhm Water.Crystal Clear, Reproducible Gels. Catalog number: EC-892. The use of National Diagnostics' ProtoGel Resolving Buffer will ensure the purity and performance of your Laemmli gels. When diluted as directed ProtoGel Resolving Buffer forms a gel of 0.375 M Tris-HCl and 0.1% SDS, pH 8.8. Storage: ProtoGel Resolving Buffer is stable for 24 months when stored tightly capped in a dark area at room temperature.
Rockland Immunochemicals 10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED - MB-006
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.
Bioworld TT Buffer, Ready-Pack (10X), 1 L
Tris-Tricine BufferTris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more conventionally used buffers. TT Buffer allows for optimal resolution of low molecular weight proteins and elimantes the glycine front often found in gel utilizing TG-SDS and other such beffers. This improved running buffer allows for resolution of peptides as low as 5kD. TT buffer is also excellent for protein electrophoresis where resolved products will be transferred to a nylon membrane. Unlike glycine, tricine will not interfere with membrane protein sequencing. The 10X pack just requires the addition of distilled H2O to make 1.0 Liter of 10X liquid. A simple 10-fold dilution with water provides a 1X solution, that makes an excellent running buffer for most applications. Technical Information:Ultra PureGradeStorage: RTHazard: Irritant
eMolecules tert-butyl 3-(hydroxymethyl)-3-methylazetidine-1-carboxylate | 1363382-91-5 | MFCD20926177 | 1g
Pharmablock | tert-butyl 3-(hydroxymethyl)-3-methylazetidine-1-carboxylate | 1g | 551132908 | PBN20120070 | | 1363382-91-5 | MFCD20926177 | 201.266 | C10H19NO3
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool